NGS(RNA-Seq)

We offer single cell RNA-Seq service to analyze transcriptome of single cell for overcome the limit of whole transcriptome analysis of cell or tissue. Through this service, we can provide researchers with cell type identities, gene expression diversity of each cell and various data analysis such as UMAP, featrue selection, clustering et al..

Sample requirement++	- Cell line : ~1x106 cells in 14ml media(Cell viability >90%) - Primary cell or FACS sorted cell : 250,000 cells in 500 ul media (Viability >70%)
Library method	10X Genomics Next GEM technology
NGS run format	Nova-Seq 6000, PE100bp
Data yield++	~60,000 reads(30,000 Paired Reads) per cell
Number of Target cell++	~5,000 cells
Turnaround time	~4 weeks after Cell counting & QC
Sample type	Cell, Nuclei

++ We can change start cell number, target cell number and sequencing depth after discussing with customer.

We support various data analysis such as gene expression data, UMAP, tSNE, feature selection, clustering heatmap, PCA, cluster biomarkers, cell type annotation, trajectory inference, cell-cell interaction, et al. with Cell Ranger, Loupe, Seurat, ExSCERA and other programs.

Single Cell Immune Profiling service provides a comprehensive approach to simultaneously examine cellular heterogeneity of the immune system, T- and B-cell repertoire diversity, and antigen specificity at single cell resolution. Through this service, we can provide researchers with cell type identities, gene expression diversity of each cell and various data analysis such as UMAP, Feature selection, Violin Plot. In addition, we support a variety of analyses such as clonotype determination by cell type, clonotype frequency for TCR and BCR, Full-length V(D)J gene sequences and heavy-light chain & α-β chain pairing.

Sample requirement++	- Cell line : ~1x106 cells in 1.5ml tube with media (Cell viability >90%) - Primary cell of FACS sorted cell : 250,000 cells in media (Cell viability >70%)
Library method	10X Genomics Next GEM technology
NGS run format	Nova-Seq 6000, PE100bp
Data yield++	* Minimum 15,000 reads pairs per cell for V(D)J library * Minimum 50,000 reads pairs per cell for 5’ Gene Expression library
Number of Target cell++	~10,000 cells
Turnaround time	~4 weeks after Cell counting & QC
Sample type	Cell

++ We can change start cell number, target cell number and sequencing depth after discussing with customer.

We support various data analysis such as gene expression data, UMAP, t-SNE, feature selection, clustering heatmap, PCA, clustering heatmap, PCA, cluster biomarkers, cell type annotation, clonotype frequency for TCR and BCR, Full-length V(D)J gene sequences and heavy-light chain & α-β chain pairing.

GeoMx Spatial Transcriptome Service combines the best of spatial and molecular profiling technologies by generating a whole tissue image at single cell resolution and digital profiling data. This unique combination of high-plex, high-throughput spatial profiling enables researchers to rapidly and quantitatively assess the biological implications of the heterogeneity within tissue samples.

Service Name	GeoMx Spatial WTA* Service	GeoMx Spatial CTA* Service
Species	Human, Mouse	Human
Number of Gene	Human ~18,000ea / Mouse ~20,000ea	~1,800ea
Data yield	~100 reads/㎛2	~30 reads/㎛2
Sample type	Tissue (FFPE, Tissue microarray, Fresh frozen tissue, Core needle biopsy etc,.)
Sample size	14.6 x 36.2 mm
Turnaround time	~3 weeks after ROI selection
Publication	NanoString GeoMx Publication

*WTA (Whole Transcriptome Atlas) / *CTA (Cancer Transcriptome Atlas)

We support various data analysis such as connects Gene expression data(ExDEGA report) with spatial information. Also we provide visualization include clusters, heatmaps, volcano plots, bar graphs, box plots and scatter plots.

We offer transcriptome analysis by extracting RNA from biological species done with Genome Sequencing and applying NGS technology. In addition to mRNA expression profiling analysis, we support various analyses which include lncRNA, isoform, INDEL, Alternative Splicing Event and Gene fusion.

	total RNA-Seq	mRNA-Seq
Sample requirement	>2ug total RNA	>2ug total RNA
Library method	RiboCop rRNA Depletion kit + CORALL RNA-seq Library Prep kit	Poly(A) RNA Selection kit + CORALL RNA-seq Library Prep kit
NGS run format	HiSeq or NovaSeq, PE100	HiSeq or NovaSeq, PE100
Data yield	>6Gb/sample	>4Gb/sample
Turnaround time	~4 weeks after RNA QC	~4 weeks after RNA QC
Sample type	Tissue, Cell, Whole Blood(Paxgene), Plant, Fungi, Bacteria, etc.

++ If you want to get RNA prep.service, you can send us your samples after discussing with us.

	Basic Analysis			Optional Analysis
	Lnc RNA	mRNA Expression	Isoform Expression	INDEL/SNP Analysis	Splicing Variant	Gene Fusion
Total RNA-Seq	O	O	O	O	O	O
mRNA-Seq		O	O	O	O	O

We support various analysis such as ExDEGA Report, GO, Pathway, Clustering, Network, GSEA, Data Mining et cetera..

We provide service of performing sequencing of 3’ UTR of RNA extracted from biological species with Poly(A) tail and analyzing gene expression. We provide high quality data with a low cost when only mRNA expression profiling analysis is requested.

Sample requirement	>10ng total RNA
Library method	QuantSeq 3' mRNA-Seq Library Prep Kit FWD
NGS run format	NextSeq 500/550, SE 75bp
Data yield	>10M read/sample
Turnaround time	~3 weeks after RNA QC
Sample type	Tissue, Cell, Whole Blood(Paxgene), Plant, Fungi, FFPE, Sorted cell, etc.

++ If you want to get RNA prep.service, you can send us your samples after discussing with us.

We support various analysis such as ExDEGA Report (mRNA expression), GO, Pathway, Clustering, Network, GSEA, Data Mining et cetera..

Bacteria RNA-Seq is an important factor in data quality by efficiently removing only rRNA from total RNA. We can remove bacterial rRNA effectively using single-stranded DNA probes and provide customer with reliable transciptome analysis through NGS technology.

Sample requirement	>2ug total RNA
Library method	RiboCop rRNA Depletion kit + CORALL RNA-seq Library Prep kit
NGS run format	HiSeq or NovaSeq, PE100(Paired End 100bp)
Data yield	>~2Gb (Data yield is varied depending on the species)
Turnaround time	~4 weeks after RNA QC
Available species	Genome sequencing completed prokaryotes on NCBI DB (Species Search)

++ If you want to get RNA prep.service, you can send us your samples after discussing with us.

We support various analysis such as ExDEGA Report (mRNA expression), GO, Pathway, Clustering, Network, GSEA, Data Mining et cetera..

We analyze, through NGS Sequencing, the amount of small-sized RNA such as microRNA and piRNA that exist in cell, tissue, exosome, and body fluid. Based on such database, we perform analysis of target gene and various kinds of data.

Sample requirement	>2ug total RNA (Tissue, Cell) >20ng RNA (Exosome, Bodyfluid)
Library method	NEBNext Multiplex Small RNA Library Prep Kit
NGS run format	NextSeq 500/550, SE 75bp
Data yield	>20M read/sample
Turnaround time	~4 weeks after RNA QC
Sample type	Tissue, Cell, Whole Blood(Paxgene), Serum, Plasma, Urine, Exosome, etc.

++ If you want to get RNA prep.service, you can send us your samples after discussing with us.

We support various analysis such as ExDEGA Report (miRNA expression), piRNA profiling, GO, Pathway, Clustering, Network, Target gene, Data Mining et cetera..